Journal
EXPERIMENTAL NEUROLOGY
Volume 168, Issue 1, Pages 183-191Publisher
ACADEMIC PRESS INC
DOI: 10.1006/exnr.2000.7593
Keywords
Parkinson's disease; human fetal tissue; 6-OHDA; transplantation; IGF-I; bFGF
Categories
Funding
- NCRR NIH HHS [M01 RR00069] Funding Source: Medline
- NINDS NIH HHS [R01 NS38619, R01 NS18639, R01 NS29982] Funding Source: Medline
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To promote dopamine cell survival in human fetal tissue strands transplanted into immunosuppressed 6-OHDA-lesioned rats, we have preincubated tissue in insulin-like growth factor-I (IGF-I, 150 ng/ml) and basic fibroblast growth factor (bFGF, 15 ng/ml) in vitro for 2 weeks. Growth factor treatment did not affect the rate of homovanillic acid production in vitro but increased overall dopamine neuron survival in animals after transplant from 1240 +/- 250 to 2380 +/- 440 neurons (P < 0.05). Animals in the growth factor-treated group had a significantly greater reduction in methamphetamine-induced rotation (66%) compared to control transplants (30%, P < 0.05). We conclude that in vitro preincubation of human fetal tissue strands with IGF-I and bFGF improves dopamine cell survival and the behavioral outcome of transplants. (C) 2001 Academic Press.
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