Journal
SEED SCIENCE RESEARCH
Volume 11, Issue 1, Pages 45-54Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1079/SSR200059
Keywords
beta-tubulin; BrdU; cell cycle; cytoskeleton; DNA synthesis; dormancy; Lycopersicon esculentum
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Cell cycle activities are initiated following imbibition of non-dormant seeds. However, it is not known whether cell cycle related events other than DNA replication also remain suppressed in imbibed dormant seeds. The objective of this study was to demonstrate that the transitions between the non-dormant and dormant (both primary and secondary) states are reflected in cell cycle events, such as DNA replication and the changing patterns of the microtubular cytoskeleton involved in the processes of growth and development. The present studies were conducted on seeds from tomato (Lycopersicon esculentum cv. Moneymaker) that possessed primary dormancy or were manipulated to attain secondary dormancy. In addition, a non-dormant abscisic acid (ABA)-deficient mutant, sit(w), was used. DNA replication, as measured by flow cytometry, acid beta -tubulin accumulation, analysed by immunoblotting, were compared with immunocytological studies of active DNA synthesis and microtubular cytoskeleton formation. It is shown that the depth of dormancy, which distinguishes primary and secondary dormancy, may depend on the progression of the cell cycle prior to the induction of dormancy.
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