Journal
BIOLOGICAL CHEMISTRY
Volume 382, Issue 3, Pages 495-498Publisher
WALTER DE GRUYTER & CO
DOI: 10.1515/BC.2001.062
Keywords
diode lasers; fluorescent labels; life time; quantum yield
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Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.
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