Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1820, Issue 2, Pages 84-89Publisher
ELSEVIER
DOI: 10.1016/j.bbagen.2011.11.006
Keywords
Human Sertoli cell; Energy metabolism; Insulin; Lactate; Glucose
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/72733/2010, SFRH/BPD/80451/2011]
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Background: Sertoli cells metabolize glucose producing lactate for developing germ cells. As insulin regulates glucose uptake and its disturbance/insensitivity is associated with diabetes mellitus, we aimed to determine the effect of insulin deprivation in human Sertoli cell (hSC) metabolism and metabolism-associated gene expression. Methods: hSC-enriched primary cultures were maintained in the absence/presence of insulin and metabolite variations were determined by H-1-NMR. mRNA expression levels of glucose transporters (GLUT1, GLUT3), lactate dehydrogenase (LDHA) and monocarboxylate transporter (MCT4) were determined by RT-PCR. Results: Insulin deprivation resulted in decreased lactate production and in decrease of glucose consumption that was completely reverted after 6 h. Cells of both groups consumed similar amounts of glucose. In insulin-deprived cells, transcript levels of genes associated to lactate metabolism (LDHA and MCT4) were decreased. Transcript levels of genes involved in glucose uptake exhibited a divergent variation: GLUT3 levels were decreased while GLUT1 levels increased. Insulin-deprived hSCs presented: 1) altered glucose consumption and lactate secretion; 2) altered expression of metabolism-associated genes involved in lactate production and export; 3) an adaptation of glucose uptake by modulating the expression of GLUT1 and GLUT3. General significance: This is the first report regarding the effect of insulin-deprivation on hSC metabolism. (C) 2011 Elsevier B.V. All rights reserved.
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