Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 91-3, Issue -, Pages 469-478Publisher
HUMANA PRESS INC
DOI: 10.1385/ABAB:91-93:1-9:469
Keywords
starch; hydrolysis; phosphoric acid; maltodextrinization; astaxanthin; byproducts
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Kinetic conditions were established for the depolymerization of cassava starch for the production of maltodextrins and glucose syrups. Thin-layer chromatography and high-performance liquid chromatography analyses corroborated that the proper H,PO, strength and thermopressurization range (e.g., 142-170 degreesC; 2.8-6.8 atm) can be successfully explored for such hydrolytic purposes of native starch granules. Because phosphoric acid can be advantageously maintained in the hydrolysate and generates, after controlled neutralization with ammonia, the strategic nutrient triplet for industrial fermentations (C, P, N), this pretreatment strategy can be easily recognized as a recommended technology for hydrolysis and upgrading of starch and other plant polysaccharides. Compared to the classic catalysts, the mandatory desalting step (chloride removal by expensive anion-exchange resin or sulfate precipitation as the calcium-insoluble salt) can be avoided. Furthermore, properly diluted phosphoric acid is well known as an allowable additive in several popular soft drinks such as colas since its acidic feeling in the mouth is compatible and synergistic with both natural and artificial sweeteners. Glycosyrups from phosphorolyzed cassava starch have also been upgraded to high-value single-cell protein such as the pigmented yeast biomass of Xanthophyllomyces dendrorhous (Phaffia rhodozyma), whose astaxanthin (wiketo-dihydroxy-beta -carotene) content may reach 0.5-1.0 mg/g of dry yeast call. This can he used as an ideal complement for animal feeding as well as a natural staining for both fish farming (meat) and poultry (eggs).
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