Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1790, Issue 1, Pages 65-70Publisher
ELSEVIER
DOI: 10.1016/j.bbagen.2008.08.007
Keywords
Q beta phage; RNA phage; Infection dynamics of bacteriophage
Categories
Funding
- Special Coordination Funds for Promoting Science and Technology: Yuragi Project
- Ministry of Education, Culture, Sports, Science and Technology, Japan
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In this study, the infection cycle of bacteriophage W was investigated. Adsorption of bacteriophage Q beta to Escherichia coli is explained in terms of a collision reaction, the rate constant of which was estimated to be 4 x 10(-10) ml/cells/min. In infected cells, approximately 130 molecules of beta-subunit and 2 x 10(5) molecules of coat protein were translated in 15 min. Replication of Q beta RNA proceeded in 2 steps-an exponential phase until 20 min and a non-exponential phase after 30 min. Prior to the burst of infected cells, phage RNAs and coat proteins accumulated in the cells at an average of up to 2300 molecules and 5x10(5) molecules, respectively. An average of 90 infectious phage particles per infected cell was released during a single infection cycle up to 105 min. (C) 2008 Elsevier B.V. All rights reserved.
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