Journal
BRITISH JOURNAL OF PHARMACOLOGY
Volume 132, Issue 6, Pages 1201-1208Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjp.0703925
Keywords
P2X receptors; artery; smooth muscle; calcium
Categories
Ask authors/readers for more resources
1 Contractile responses to short trains of nerve stimulation have been characterized in small, medium and large arteries from the rat mesenteric circulation (5th-6th, 2nd-3rd and 1st order, respectively). In addition, sources of calcium for smooth muscle contraction have been investigated. 2 Nerve stimulation (10 pulses at 10 Hz) evoked reproducible contractions. The P2 receptor antagonist suramin (100 muM) reduced constrictions by 65.3 +/- 7.4, 82.7 +/- 3.3 and 3.1 +/- 6.1% in small, medium and large arteries respectively. The alpha -adrenoceptor antagonist prazosin (0.1 muM) reduced responses by 32.6 +/- 2.6, 27.0 +/- 1.5 and 97.0 +/- 1.9% respectively. 3 The L-type calcium channel antagonist nifedipine (1 muM) reduced nerve-evoked contractions by 2.8 +/- 3.3, 10.0 +/- 3.7 and 13.5 +/- 2.7% in small, medium and large arteries respectively. When the adrenergic component of contraction was blocked by prazosin (0.1 muM) nifedipine reduced responses by 4.6 +/- 7.9, 14.3 +/- 2.0 and 3.0 +/- 1.9% respectively. 4 Contractile responses to exogenous alpha,beta -meATP were unaffected by the depletion of calcium stores with cyclopiazonic acid (30 muM). This indicates that mobilization of calcium from internal stores is not required for P2X receptor mediated smooth muscle contraction. 5 We conclude that for neurogenic responses, the P2X receptor mediated component of constriction dominates in small mesenteric arteries (3rd -6th order) while in large arteries (1st order) noradrenaline mediates contraction. For P2X receptor mediated responses all the calcium required for smooth muscle contraction enters the cell directly through P2X receptor channels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available