Journal
EMBO JOURNAL
Volume 20, Issue 5, Pages 1144-1152Publisher
OXFORD UNIV PRESS
DOI: 10.1093/emboj/20.5.1144
Keywords
chloroplast; in vitro system; RNA-binding protein; RNA editing; trans-acting factor
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RNA editing in higher plant chloroplasts involves C --> U conversion at similar to 30 specific sites. An irt vitro system supporting accurate editing has been developed from tobacco chloroplasts. Mutational analysis of substrate mRNAs derived from tobacco chloroplast psbL and ndhB mRNAs confirmed the participation of cis-acting elements that had previously been identified in vivo. Competition analysis revealed the existence of site-specific trans-acting factors interacting with the corresponding upstream cis-elements. A chloroplast protein of 25 kDa was found to be specifically associated with the cis-element involved in psbL mRNA editing. Immunological analyses revealed that an additional factor, the chloroplast RNA-binding protein cp31, is also required for RNA editing at multiple sites. This combination of site-specific and common RNA-binding proteins recognizes editing sites in chloroplasts.
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