Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1860, Issue 12, Pages 2499-2505Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2018.09.009
Keywords
Articular chondrocyte; Cartilage; Bone cells; Synovial cells; Joint; connexin43; Gap junctions; Cellular communication; Osteoarthritis
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Funding
- Spanish Society for Rheumatology, SER (FER2013)
- Spanish Society for Research on Bone and Mineral Metabolism, SEIOMM (FEIOMM2016)
- Xunta de Galicia [IN607B 2017/21]
- Health Institute Carlos III (ISCIII, Spain) [PI16/00035]
- European Regional Development Funds, A way of making Europe from the European Union
- National Institutes of Health [R01GM088181]
- Universidade de A Coruna
- Deputacion de A Coruna
- Fundacion Barrie
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Cell-to-cell communication between bone, cartilage and the synovial membrane is not fully understood and it is only attributed to the diffusion of substances through the extracellular space or synovial fluid. In this study, we found for the first time that primary bone cells (BCs) including osteocytes, synovial cells (SCs) and chondrocytes (CHs) are able to establish cellular contacts and to couple through gap junction (GJ) channels with connexin43 (Cx43) being dominant. Transwell co-culture and identification by mass spectrometry revealed the exchange of essential amino acids, peptides and proteins including calnexin, calreticulin or CD44 antigen between contacting SCs, BCs and CHs. These results reveal that CHs, SCs and BCs are able to establish intercellular connections and to communicate through GJ channels, which provide a selective signalling route by the direct exchange of potent signalling molecules and metabolites.
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