Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1838, Issue 1, Pages 413-418Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2013.10.007
Keywords
Amphipathic helix; Membrane active peptide; Twin arginine transport; Chloroplast TatB
Categories
Funding
- DOE Office of Science Early Career Research Program grant [DE-FG02-10ER16138]
- Office of Basic Energy Sciences and National Institutes of Health [R01GM080542, GM080542Z]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1305664] Funding Source: National Science Foundation
Ask authors/readers for more resources
The chloroplast twin arginine translocation (cpTat) system transports highly folded precursor proteins into the thylakoid lumen using the protonmotive force as its only energy source. Hcfl 06, as one of the core components of the cpTat system, is part of the precursor receptor complex and functions in the initial precursor-binding step. Hcfl 06 is predicted to contain a single amino terminal transmembrane domain followed by a Pro-Gly hinge, a predicted amphipathic cc-helix (APH), and a loosely structured carboxy terminus. Hcf106 has been shown biochemically to insert spontaneously into thylakoid membranes. To better understand the membrane active capabilities of Hcf106, we used solid-state NMR spectroscopy to investigate those properties of the APH. In this study, synthesized peptides of the predicted Hcf106 APH (amino acids 28-65) were incorporated at increasing mol.% into 1-palmitoy1-2-oleoyl-sn-glycero-phosphocholine (POPC) and POPC/ MGDG (monogalactosyldiacylglycerol; mole ratio 85:15) multilamellar vesicles (MLVs) to probe the peptidelipid interaction. Solid-state 31P NMR and 2H NMR spectroscopic experiments revealed that the peptide perturbs the headgroup and the acyl chain regions of phospholipids as indicated by changes in spectral lineshape, chemical shift anisotropy (CSA) line width, and 2H order Scp parameters. In addition, the comparison between POPCMLVs and POPC/MGDG MLVs indicated that the lipid bilayer composition affected peptide perturbation of the lipids, and such perturbation appeared to be more intense in a system more closely mimicking a thylakoid membrane. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available