Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1828, Issue 1, Pages 23-34Publisher
ELSEVIER
DOI: 10.1016/j.bbamem.2012.02.028
Keywords
Connexin; Cx43; Gap junction; J; Phosphorylation; Proteomic; Multisite phosphorylation
Categories
Funding
- Canadian Institutes for Health Research
- Heart and Stroke Foundation of BC and Yukon
- Canadian Stroke Network
- Heart and Stroke Foundation of Canada/BC and Yukon
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Connexins require an integrated network for protein synthesis, assembly, gating, internalization, degradation and feedback control that are necessary to regulate the biosynthesis, and turnover of gap junction channels. At the most fundamental level, the introduction of sequence-altering, modifications introduces changes in protein conformation, activity, charge, stability and localization. Understanding the sites, patterns and magnitude of protein post-translational modification, including phosphorylation, is absolutely critical. Historically, the examination of connexin phosphorylation has been placed within the context that one or small number of sites of modification strictly corresponds to one molecular function. However, the release of high-profile proteomic datasets appears to challenge this dogma by demonstrating connexins undergo multiple levels of multi-site phosphorylation. With the growing prominence of mass spectrometry in biology and medicine, we are now getting a glimpse of the richness of connexin phosphate signals. Having implications to health and disease, this review provides an overview of technologies in the context of targeted and discovery proteomics, and further discusses how these techniques are being applied to fill the gaps in understanding of connexin post-translational control. This article is part of a Special Issue entitled: The Communicating junctions, roles and dysfunctions. (c) 2012 Elsevier B.V. All rights reserved.
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