Journal
FISH PATHOLOGY
Volume 36, Issue 1, Pages 7-11Publisher
JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR
DOI: 10.3147/jsfp.36.7
Keywords
Flavobacterium psychrophilum; in situ hybridization; ayu; Plecoglossus altivelis; bacterial coldwater disease; detection
Categories
Ask authors/readers for more resources
An in situ hybridization (ISH) using digoxigenin-labeled 16S rDNA probes was developed to detect the causative agent of bacterial coldwater disease, Flavobacterium psychrophilum. Strong, specific hybridization was observed for all F. psychrophilum strains tested and no crosshybridization was seen for 13 other bacterial species. Distribution of F. psychrophilum in various organs of experimentally and naturally infected ayu was investigated by the ISH. A group of the experimental fish were given a sting at the skin and then immersed in a suspension of F. psychrophilum (10(6) CFU/mL) for 1 h at 15 degreesC and another group were subcutaneously injected with F. psychrophilum at a dose of 1.5x10(7) CFU/fish. In both experiments, F. psychrophilum was detected in the muscle, gills, heart, kidney and spleen, but not in the brain, liver, stomach, intestine, or pyloric caeca during the experiment period (7 h). A large number of F. psychrophilum were found at the stung or injected sites of the muscle, while no bacteria were detected at the intact muscle. In some of naturally infected ayu collected in fish farms, F. psychrophilum was detected in the liver, pancreas, stomach, pyloric caeca and intestine. The muscle of the naturally infected ayu was severely infected with F. psychrophilum, but no bacterium was detected in the brain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available