Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1798, Issue 12, Pages 2182-2189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.11.011
Keywords
Cell-penetrating peptide; Drug delivery; MALDI-TOF MS; Internalization efficiency; Intracellular degradation; Thiol/disulfide exchanges
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Funding
- Agence Nationale pour la Recherche
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This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms. (C) 2009 Elsevier B.V. All rights reserved.
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