Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1788, Issue 10, Pages 2124-2131Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.07.017
Keywords
Coiled-coil; Fluorescence imaging; Membrane protein; Tag-probe labeling
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Funding
- MEXT, Japan
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Instead of using reconstituted proteoliposomes, in situ investigations of membrane proteins in living cell membranes are important because the heterogeneous and dynamic nature of biomembranes significantly affects their behavior. Protein-specific labeling is a key technique for the detection of a target protein by fluorescence measurements, particularly fluorescence microscopy. However, conventional genetic fusion with fluorescent proteins has several shortcomings. Post-translational labeling methods using a genetically encodable tag and synthetic probes targeting to the tag can overcome these limitations. This review summarizes emerging tag-probe techniques for labeling specific membrane proteins and their applications, including endocytotic internalization, partitioning to specific membrane domains, interprotein interactions, and conformational changes. (C) 2009 Elsevier B.V. All rights reserved.
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