Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1788, Issue 5, Pages 911-917Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.03.003
Keywords
Amiodarone; OATP2B1; Alveolar epithelial type II; AIPT
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The accumulation mechanisms of amiodarone (AMD) involving transporters in lung alveolar epithelial type 11 cells were studied. The uptake of AMD was examined using human alveolar epithelia I-derived cell line A549 as a model. AMD was transported by the carrier-mediated system, and the apparent K-m and V-max values were 66.8 +/- 30.3 mu M and 49.7 +/- 9.7 nmol/mg protein/5 min, respectively. The uptake of AMD by A549 cells was Na+-independent and was inhibited by substrates of human organic anion transporting polypeptide (OATP). The inhibition profiles were similar to the inhibitory effects of several compounds on OATP2B1 -mediated E-3-S transport, and RT-PCR analysis showed mRNA expression of OATP2B1 and 1B3 in A549 cells. SiRNAs targeted to the OATP2B1 gene decreased the OATP2B1 mRNA expression level in A549 cells up to about 50% and reduced the uptake of AMD up to about 40%. These results indicate that AMD uptake mediated by carriers, including OATP2B1, might lead to accumulation of AMD in the lung and AMD-induced pulmonary toxicity (AIPT). (C) 2009 Elsevier B.V. All rights reserved.
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