Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 30, Issue 5, Pages 463-488Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0891-5849(00)00373-7
Keywords
nitric oxide; superoxide; peroxynitrite; hydroxyl radical; nitrogen dioxide; 3-nitrotyrosine; free radicals; detection; methods
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Peroxynitrite promotes oxidative damage and is implicated in the pathophysiology of various diseases that involve accelerated rates of nitric oxide and superoxide formation. The unambiguous detection of peroxynitrite in biological systems is, however, difficult due to the combination of a short biological half-life, limited diffusion, multiple target molecule reactions, and participation of alternative oxidation/nitration pathways. In this review, we provide the conceptual framework and a comprehensive analysis of the current experimental strategies that can serve to unequivocally define the existence and quantitation of peroxynitrite in biological systems of different levels of organization and complexity. (C) 2001 Elsevier Science Inc.
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