Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1837, Issue 10, Pages 1674-1683Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2014.05.353
Keywords
EF4(LepA/GUF1); C elegans; Mitochondrial dysfunction; Retrograde pathways; Translation
Categories
Funding
- National Key Basic Research Program of China/973 Program [2013CB531200, 2012CB911001]
- National Natural Science Foundation of China [31170756, 31270847, 31322015, 31000596]
- CAS [KSZD-EW-Z-003]
- National Laboratory of Biomacromolecules in Institute of Biophysics
- Shanghai Key Laboratory of Molecular Andrology
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How animals coordinate cellular bioenergetics in response to stress conditions is an essential question related to aging, obesity and cancer. Elongation factor 4 (EF4/LEPA) is a highly conserved protein that promotes protein synthesis under stress conditions, whereas its function in metazoans remains unknown. Here, we show that, in Caenorhabditis elegans, the mitochondria-localized CeEF4 (referred to as mtEF4) affects mitochondrial functions, especially at low temperature (15 degrees C). At worms' optimum growing temperature (20 degrees C), mtef4 deletion leads to self-brood size reduction, growth delay and mitochondrial dysfunction. Transcriptomic analyses show that mtef4 deletion induces retrograde pathways, including mitochondrial biogenesis and cytoplasmic translation reorganization. At low temperature (15 degrees C), mtef4 deletion reduces mitochondrial translation and disrupts the assembly of respiratory chain supercomplexes containing complex IV. These observations are indicative of the important roles of mtEF4 in mitochondrial functions and adaptation to stressful conditions. (C) 2014 Elsevier B.V. All rights reserved.
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