Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1817, Issue 10, Pages 1879-1885Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2012.03.033
Keywords
Respiratory complex 1; Dihydrolipoamide dehydrogenase; Hydrogen peroxide; Pyridine nucleotides; Mitochondria
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Funding
- Russian Foundation for Fundamental Research [11-04-00916, 12-04-00602]
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The rates of NADH-supported superoxide/hydrogen peroxide production by membrane-bound bovine heart respiratory complex I. soluble pig heart dihydrolipoamide dehydrogenase (DLDH), and by accompanying operation of these enzymes in rat heart mitochondrial matrix were measured as a function of the pool of pyridine nucleotides and its redox state. Each of the activities showed nontrivial dependence on nucleotide pool concentration. The NAD(+)/NADH ratios required for their half maximal capacities were determined. About half of the total NADH-supported H2O2 production by permeabilized mitochondria in the absence of stimulating ammonium could be accounted for by DLDH activity. The significance of the mitochondrial NADH-dependent hydrogen peroxide production under physiologically relevant conditions is discussed. This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012). (C) 2012 Elsevier B.V. All rights reserved.
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