Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1817, Issue 3, Pages 410-418Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2011.12.009
Keywords
Brown adipose tissue; Mitochondria; Fatty acid oxidation; Reactive oxygen species (ROS); Reverse electron transfer
Categories
Funding
- Kultusministerium of Sachsen-Anhalt
Ask authors/readers for more resources
Mitochondria from brown adipose tissue (BATM) have a high enzymatic capacity for fatty acid oxidation and therefore are an ideal model to examine the sites of reactive oxygen species (ROS) generation during fatty acid oxidation. ROS generation by BATM (isolated from 3-week-old rats) was measured during acylcarnitine oxidation as release of H2O2 into the medium and as inactivation of the matrix enzyme aconitase. The following results were obtained: (1) BATM release large amounts of H2O2 in the coupled as well as in the uncoupled states, several times more than skeletal muscle mitochondria. (2) H2O2 release is especially large with acyl-carnitines of medium-chain fatty acids (e.g. octanoylcarnitine). (3) Reverse electron transport does not contribute in a significant extent to the overall ROS generation. (4) Despite the large release of H2O2, the ROS-sensitive matrix enzyme aconitase is not inactivated during acylcarnitine oxidation. (5) In contrast to acylcarnitines, oxidation of alpha-glycerophosphate by BATM is characterized by large H2O2 release and a pronounced aconitase inactivation. We hypothesize that acylcarnitine-supported ROS generation in BATM may be mainly associated with acyl-CoA dehydrogenase and electron transferring flavoprotein-ubiguinone reductase rather than with complexes of the respiratory chain. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available