Journal
ANALYST
Volume 140, Issue 2, Pages 654-660Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an01324g
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Funding
- University of Navarra (Spain)
- Secretary of State of Investigation, Development and Innovation of the Ministry of Economy and Competitivity of Spain [DPI 2012-38090-C03-D3]
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This paper describes the design, implementation and validation of a sensitive and integral technology solution for endotoxin detection. The unified and portable platform is based on the electrochemical detection of endotoxins using a synthetic peptide immobilized on a thin-film biosensor. The work covers the fabrication of an optimized sensor, the biofunctionalization protocol and the design and implementation of the measuring and signalling elements (a microfluidic chamber and a portable potentiostat-galvanostat), framed ad hoc for this specific application. The use of thin-film technologies to fabricate the biosensing device and the application of simple immobilization and detection methods enable a rapid, easy and sensitive technique for in situ and real time LPS detection.
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