Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 104, Issue 4, Pages 833-841Publisher
WILEY
DOI: 10.1002/jbm.a.35622
Keywords
radius defect model; cell-free scaffold; matrix stiffness; cell recruitment; osteogenic differentiation
Funding
- National Natural Science Foundation of China [11172338]
- Fundamental Research Funds for the Central Universities [CQDXWL-2012-Z001]
- Innovation and Attracting Talents Program for College and University ('111' Project) [B06023]
- Sharing Fund of Chongqing University's Large-scale Equipment
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To promote bone healing, bone repair biomaterials are increasingly designed to incorporate growth factors. However, the impact of matrix mechanics of cell-free scaffold independent of microstructure on the osteogenic differentiation of endogenous osteoprogenitor cells orchestrating bone repair and regeneration remains not to be fully understood. In our recent study, three-dimensional (3D) scaffolds with different stiffness but same microstructure have been successfully fabricated by coating decellularized bone with collagen/hydroxyapatite (HA) mixture with different collagen rations. It has been demonstrated that the scaffold with optimal stiffness can induce the osteogenic differentiation of MSCs in vitro and in the subcutaneous tissue. The present in vivo study further investigated the repair efficiency of these scaffolds in a rabbit radius with a critical-sized segmental defect model and its potential mechanism. Micro-computed tomography (-CT), X-ray and histological analysis were carried out to evaluate the repair capacity of these scaffolds. The results demonstrated that the cell-free scaffold with optimal stiffness incorporation of endogenous osteoprogenitor cells significantly promoted the repair and reconstruction quality of mass bone defect. One of the crucial mechanisms was that hypoxia and stromal cell-derived factor-1 (SDF-1) mediated mesenchymal stem cells (MSCs) migration by which matrix mechanics exerted influence on bone fracture healing. These findings suggested that only modulating the matrix stiffness of cell-free scaffold can be one of the most attractive strategies for promoting the progression of bone healing. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 833-841, 2016.
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