Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 104, Issue 1, Pages 313-321Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.35565
Keywords
smooth muscle cells; tissue engineering; vascular graft; tunica media; cell migration
Funding
- Surrey-NPL Partnership
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Crosslinked, multi-layer electrospun gelatin fiber scaffolds with generally +/- 45 degree fiber orientation have been used to grow human umbilical vein smooth muscle cells (HUVSMCs) to create a vascular tunica media graft. Scaffolds of different fiber diameter (2-5 m in wet state), pore size, and porosity (16-21% in wet state) were assessed in terms of cell adherence and viability, cell proliferation, and migration in both in-plane and transverse directions through the scaffold as a function of time under static cell culture conditions. HUVSMC cell viability reached between 80 and 92% for all scaffolds after 9 days in culture. HUVSMCs adhered, elongated, and orientated in the fiber direction, and migrated through a scaffold thickness of 200-235 m 9 days post-seeding under static conditions. The best scaffold was then used to assess the tissue engineering of HUVSMCs under dynamic conditions for a rotating, cell seeded, tubular scaffold in the bioreactor containing the culture medium. Dynamic conditions almost doubled the rate of cell proliferation through the scaffold, forming full tissue throughout a scaffold of 250-300 m thickness 6 days post-seeding. (c) 2015 Wiley Periodicals, Inc.
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