Mitochondria-cytoskeleton interaction: Distribution of β-tubulins in cardiomyocytes and HL-1 cells

Mitochondria-cytoskeleton interactions were analyzed in adult rat cardiomyocytes and in cancerous non-beating HL-1 cells of cardiac phenotype. We show that in adult cardiomyocytes beta II-tubulin is associated with mitochondrial outer membrane (MOM). beta I-tubulin demonstrates diffused intracellular distribution, beta III-tubulin is colocalized with Z-lines and beta IV-tubulin forms microtubular network. HL-1 cells are characterized by the absence of beta II-tubulin, by the presence of bundles of filamentous beta IV-tubulin and diffusely distributed beta I- and beta III-tubulins. Mitochondrial isoform of creatine kinase (MtCK), highly expressed in cardiomyocytes, is absent in HL-1 cells. Our results show that high apparent K-m for exogenous ADP in regulation of respiration and high expression of MtCK both correlate with the expression of beta II-tubulin. The absence of beta II-tubulin isotype in isolated mitochondria and in HL-1 cells results in increased apparent affinity of oxidative phosphorylation for exogenous ADP. This observation is consistent with the assumption that the binding of beta II-tubulin to mitochondria limits ADP/ATP diffusion through voltage-dependent anion channel of MOM and thus shifts energy transfer via the phosphocreatine pathway. On the other hand, absence of both beta II-tubulin and MtCK in HL-1 cells can be associated with their more glycolysis-dependent energy metabolism which is typical for cancer cells (Warburg effect). (C) 2011 Elsevier B.V. All rights reserved.