Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1797, Issue 2, Pages 272-277Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2009.11.004
Keywords
ATP synthase; Electron tomography; Mitochondria; Polytomella
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Funding
- Netherlands Organization for Scientific Research NWO
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The fine structure of intact, close-to-spherical mitochondria from the alga Polytomella was visualized by dual-axis cryo-electron tomography. The supramolecular organization of dimeric ATP synthase in the cristae membranes was investigated by averaging subvolumes of tomograms and 3D details at similar to 6 nm resolution were revealed. Oligomeric ATP synthase is composed of rows of dimers at 12 run intervals; the dimers make a slight angle along the row. In addition, the main features of monomeric ATP synthase, such as the conically shaped F, headpiece, central stalk and stator were revealed. This demonstrates the capability of dual-axis electron tomography to unravel details of proteins and their interactions in complete organelles. (C) 2009 Elsevier B.V. All rights reserved.
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