Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 26, Issue 3, Pages 121-126Publisher
NATURE AMERICA INC
DOI: 10.1038/sj.jim.7000097
Keywords
polyhydroxybutyrate; PHB; biodegradable thermoplastics; acetic acid; Ralstonia eutropha; Alcaligenes eutrophus
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Batch cultures of Ralstonia eutropha in chemically defined media with acetic acid (HAc) as the sole carbon source were conducted to investigate acetate utilization, formation of poly(3-hydroxybutyrate) (PHB) and growth of active biomass (ABM) under different carbon to nitrogen (CIN) weight ratios. The specific acetate utilization rate based on ABM approached 0.16 g/g ABM h(-1), which was not affected very much by the extracellular HAc concentration from 1 to 5 g/l, but was affected by the CIN weight ratio. A low C/N ratio or high nitrogen supply sped up the specific acetate utilization rate to produce more ABM and less PHB. A high HAc concentration (>6 g/l), however, depressed acetate utilization as well as the ABM growth and PHB formation. A high cell mass concentration enhanced the tolerance of R, eutropha to the toxicity of HAc at pH 7 to 8.5. The viscosity-average molecular size of PHB generally increased first and then declined in batch cultures. Larger PHB molecules and less PHB per ABM were produced at a low CIN ratio with enough nutrient nitrogen than those under a high C/N ratio with less nutrient nitrogen available.
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