Journal
NATURE CELL BIOLOGY
Volume 3, Issue 3, Pages 245-252Publisher
MACMILLAN PUBLISHERS LTD
DOI: 10.1038/35060032
Keywords
-
Categories
Ask authors/readers for more resources
Amplification or overexpression of HER-2/neu in cancer cells confers resistance to apoptosis and promotes cell growth. The cellular localization of p21(Cip1/WAF1) has been proposed to be critical either in promoting cell survival or in inhibiting cell growth. Here we show that HER-2/neu-mediated cell growth requires the activation of Akt, which associates with p21(Cip1/WAF1) and phosphorylates it at threonine 145, resulting in cytoplasmic localization of p21(Cip1/WAF1). Furthermore, blocking the Akt pathway with a dominant-negative Akt mutant restores the nuclear localization and cell-growth-inhibiting activity of p21(Cip1/WAF1). Our results indicate that HER-2/neu induces cytoplasmic localization of p21(Cip1/WAF1) through activation of Akt to promote cell growth, which may have implications for the oncogenic activity of HER-2/neu and Akt.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available