Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1777, Issue 7-8, Pages 579-582Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2008.04.016
Keywords
F1-ATPase; gold nanorod; molecular motor; high-speed data acquisition; single molecule
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Funding
- NIGMS NIH HHS [R01 GM050202-11, R01 GM050202, GM50202] Funding Source: Medline
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The torque generated by the power stroke of Escherichia coli F-1-ATPase was determined as a function of the load from measurements of the velocity of the gamma-subunit obtained using a 0.25 mu s time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the gamma-subunit was able to rotate with a 20x increase in viscosity, the transition time decreased from 0.4 ms to 5.26 ms. The torque was measured to be 63 +/- 8 pN nm, independent of the load on the enzyme. (c) 2008 Elsevier B.V. All rights reserved.
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