Journal
BIOCHEMISTRY-MOSCOW
Volume 79, Issue 12, Pages 1382-1390Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S000629791412013X
Keywords
antibody; IgG; variable domain; single-domain antibody
Categories
Funding
- DWTC Belgium grant
- Russian Academy of Sciences
- Russian Foundation for Basic Research [HK-14-04-01189]
Ask authors/readers for more resources
In this work, IgG content and structures of antigen-binding domains and hinge regions of different IgG subtypes of Camelus bactrianus were analyzed in detail for the first time. Our data demonstrate that C. bactrianus contains a very large amount of heavy chain-only antibodies that can be used as a source of VHH domain-containing molecules. Despite some minor sequence differences identified in this study, C. bactrianus VHH domains possess principally the same unique features as those of C. dromedarius and the llama. These features are important for developing an efficient phage display-based anti-body selection technology. We conclude that C. bactrianus is a very suitable animal to raise an immune response that serves as a source to identify antigen-specific VHHs selected after phage display.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available