Journal
BIOCHEMISTRY-MOSCOW
Volume 77, Issue 10, Pages 1172-1180Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297912100094
Keywords
polyamines; spermine; spermidine; analogs; hepatitis C virus; RNA polymerase; helicase
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Funding
- Russian Foundation for Basic Research [10-04-00047-a, 12-04-01487-a]
- Russian Federation [MK-5035.2011.4]
- Russian Academy of Sciences
- [11.519.11.2017]
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Influence of the biogenic polyamines spermine, spermidine, and putrescine as well as their derivatives on the replication enzymes of hepatitis C virus (HCV) was investigated. It was found that spermine and spermidine activate HCV RNA-dependent RNA polymerase (NS5B protein). This effect was not caused by the stabilization of the enzyme or by competition with template-primer complex, but rather it was due to achievement of true maximum velocity V (max). Natural polyamines and their derivatives effectively inhibited the helicase reaction catalyzed by another enzyme of HCV replication - helicase/NTPase (NS3 protein). However, these compounds affected neither the NTPase reaction nor its activation by polynucleotides. Activation of the HCV RNA polymerase and inhibition of the viral helicase were shown at physiological concentrations of the polyamines. These data suggest that biogenic polyamines may cause differently directed effects on the replication of the HCV genome in an infected cell.
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