Journal
BIOCHEMISTRY-MOSCOW
Volume 76, Issue 6, Pages 636-644Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297911060034
Keywords
mycobacteria; nonculturable state; oleic acid; lipids; reactivation
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Funding
- Russian Academy of Scienc
- FTP [14.740.11.0246, P2277]
- RFBR [11-04-01440-a]
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We have found that transition of actively dividing Mycobacterium smegmatis cells into the dormant nonculturable state is accompanied by increase in the protein/lipid ratio and disappearance of one of the main lipid components of the mycobacterial cells, trehalose monomycolate. In this case, oleic acid is accumulated in the culture medium due to its secretion by the mycobacterial cells. Addition of lipids of different classes to nonculturable M. smegmatis cells induces their resuscitation. The lipid reactivating effect is evidently caused by the presence of fatty acids in their composition, because free fatty acids also exhibited reactivation effect. Oleic acid in concentration of 0.05-3 mu g/ml exhibited maximal effect, and that allows us to draw a conclusion concerning its signal role in the transition of dormant cells into active state.
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