Journal
SCIENCE
Volume 291, Issue 5510, Pages 1965-1969Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1057269
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To study the mechanisms underlying the high pathogenicity of Ebola virus, we have established a system that allows the recovery of infectious virus from cloned cDNA and thus permits genetic manipulation. We created a mutant in which the editing site of the gene encoding envelope glycoprotein (CP) was eliminated. This mutant no longer expressed the nonstructural glycoprotein sGP, Synthesis of CP increased, but most of it accumulated in the endoplasmic reticulum as immature precursor. The mutant was significantly more cytotoxic than wild-type virus, indicating that cytotoxicity caused by CP is down-regulated by the virus through transcriptional RNA editing and expression of sGP.
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