Journal
SCIENCE
Volume 291, Issue 5510, Pages 1959-1962Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1058409
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Funding
- NIGMS NIH HHS [R37 GM29169, GM60635] Funding Source: Medline
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Initiation of protein synthesis in eukaryotes requires recruitment of the 40S ribosomal subunit to the messenger RNA(mRNA). In most cases, this depends on recognition of a modified nucleotide cap on the 5' end of the mRNA. However, an alternate pathway uses a structured RNA element in the 5' untranslated region of the messenger or viral RNA called an internal ribosomal entry site (IRES). Here, we present a cryo-electron microscopy map of the hepatitis C virus (HCV) IRES bound to the 40S ribosomal subunit at about 20 Angstrom resolution. IRES binding induces a pronounced conformational change in the 40S subunit and closes the mRNA binding cleft, suggesting a mechanism for IRES-mediated positioning of mRNA in the ribosomal decoding center.
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