Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1511, Issue 1, Pages 7-16Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0005-2736(00)00355-2
Keywords
multidrug resistance-associated protein; bile salt export pump; bile acid; canalicular membrane vesicle
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Biliary excretion of certain bile acids is mediated by multidrug resistance associated protein 2 (Mrp2) and the bile salt export pump (Bsep). In the present study, the transport properties of several bile acids were characterized in canalicular membrane vesicles (CMVs) isolated from Sprague-Dawley (SD) rats and Eisai hyperbilirubinemic rats (EHBR) whose Mrp2 function is hereditarily defective and in membrane vesicles isolated from Sf9 cells infected with recombinant baculovirus containing cDNAs encoding Mrp2 and Bsep. ATP-dependent uptake of [H-3]taurochenodeoxycholate sulfate (TCDC-S) (K-m=8.8 muM) and [H-3]taurolithocholate sulfate (TLC-S) (K-m=1.5 muM) was observed in CMVs from SD rats, but not from EHBR. In addition, ATP-dependent uptake of [H-3]TLC-S (K-m=3.9 M) and [H-3]taurocholate (TC) (K-m=7.5 muM) was also observed in Mrp2 and Bsep-expressing Sf9 membrane vesicles, respectively. TCDC-S and TLC-S inhibited the ATP-dependent TC uptake into CMVs from SD rats with IC50 values of 4.6 muM and 1.2 muM, respectively. In contrast, the corresponding values for Sf9 cells expressing Bsep were 59 and 62 muM, respectively, which were similar to those determined in CMVs from EHBR (68 and 33 muM, respectively). By co-expressing Mrp2 with Bsep in Sf9 cells, IC50 values for membrane vesicles from these cells shifted to values comparable with those in CMVs from SD rats (4.6 and 1.2 muM) Moreover, in membrane vesicles where both Mrp2 and Bsep are co-expressed, preincubation with the sulfated bile acids potentiated their inhibitory effect on Bsep-mediated TC transport. These results can be accounted for by assuming that the sulfated bile acids trans-inhibit the Bsep-mediated transport of TC. (C) 2001 Elsevier Science B.V. All rights reserved.
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