4.2 Article

Design of a novel interleukin-13 antagonist from analysis of informational structure

Journal

BIOCHEMISTRY-MOSCOW
Volume 74, Issue 4, Pages 399-405

Publisher

MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297909040075

Keywords

ANIS method; informational structure; deletion variant; IL-13 antagonist; TF-1 proliferation

Funding

  1. President of the Russian Federation for Support of Leading Scientific Schools and by the Russian Academy of Sciences Program [NSh-2231.2008.4]

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Interleukin-13 (IL-13) is one of the cytokines involved in the development of Th2-type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL-13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF-1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full-length IL-13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL-13.

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