Journal
BIOCHEMISTRY-MOSCOW
Volume 73, Issue 8, Pages 862-869Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297908080038
Keywords
firefly luciferase; random mutagenesis; mutation of amino acid residues; bioluminescence spectra; pH dependence of bioluminescence spectra
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Funding
- Russian Foundation for Basic Research [08-04-00624]
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Most firefly luciferases demonstrate a strong pH-dependence of bioluminescence spectra. Gene region encoding first 225 residues of Luciola mingrelica luciferase was subjected to random mutagenesis, and four mutants with altered pH-sensitivity of bioluminescence spectra were isolated. F16L substitution showed distinctly lower pH-dependence of bioluminescence spectra, and Y35N,H and F16L/A40S substitutions resulted in the enzymes with bioluminescence spectra virtually independent from pH in the range of 6.0-7.8. The structural explanation is proposed for the effect of mutations on pH-sensitivity of bioluminescence spectra.
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