Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 387, Issue 2, Pages 209-215Publisher
ACADEMIC PRESS INC
DOI: 10.1006/abbi.2000.2256
Keywords
cartilage; matrix; matrilin; human; electrospray mass spectrometry
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Funding
- NIAMS NIH HHS [AR36794, R01 AR036794, R37 AR037318] Funding Source: Medline
- NICHD NIH HHS [HD 22657] Funding Source: Medline
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Matrilin-3 is a recently identified matrix protein of cartilage that shows sequence homology to matrilin-1 (cartilage matrix protein or CMP). Here we identify and characterize the molecular properties of matrilin-3 from human growth cartilage by immunochemical and mass spectrometry methods. Extracts of fetal skeletal cartilage were resolved by SDS-PAGE and candidate matrilin subunits were identified by electrospray mass spectrometry of tryptic peptides. Matrilin-3 and matrilin-1 were both present in disulfide-bonded tetrameric components. Polyclonal antisera to synthetic peptides specific to each subunit confirmed the identities by Western blotting and further demonstrated the existence of several forms of tetramer. A homotetramer (matrilin-3)(4) and more than one species of heterotetramer containing matrilin-3 and matrilin-1 chains were resolved. Immunohistochemistry of tissue sections confirmed that both matrilin-1 and matrilin-3 are widely codistributed throughout human skeletal growth cartilage. (C) 2001 Academic Press.
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