Journal
JOURNAL OF BIOMECHANICS
Volume 48, Issue 1, Pages 122-129Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2014.10.023
Keywords
Osteoarthritis; Articular cartilage; Resurfacing; Photocrosslinking; Mechanical properties
Categories
Funding
- National Institutes of Health - National Institute of Arthritis and Musculoskeletal and Skin Diseases [R21-AR059203, AR007281]
- National Center for Research Resources, National Institutes of Health [C06-RR12538-01]
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Surface damage to articular cartilage is recognized as the initial underlying process causing the loss of mechanical function in early-stage osteoarthritis. In this study, we developed structure-modifying treatments to potentially prevent, stabilize or reverse the loss in mechanical function. Various polymers (chondroitin sulfate, carboxymethylcellulose, sodium hyaluronate) and photoinitiators (riboflavin, irgacure 2959) were applied to the surface of collagenase-degraded cartilage and crosslinked in situ using UV light irradiation. While matrix permeability and deformation significantly increased following collagenase-induced degradation of the superficial zone, resurfacing using tyramine-substituted sodium hyaluronate and riboflavin decreased both values to a level comparable to that of intact cartilage. Repetitive loading of resurfaced cartilage showed minimal variation in the mechanical response over a 7 day period. Cartilage resurfaced using a low concentration of riboflavin had viable cells in all zones while a higher concentration resulted in a thin layer of cell death in the uppermost superficial zone. Our approach to repair surface damage initiates a new therapeutic advance in the treatment of injured articular cartilage with potential benefits that include enhanced mechanical properties, reduced susceptibility to enzymatic degradation and reduced adhesion of macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
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