Journal
TALANTA
Volume 54, Issue 1, Pages 107-113Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(00)00639-1
Keywords
arginine; chemiluminescence; flow-injection; glycine
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Some amino acids were found to react with carbonyl functional groups of humic acid. Products of this reaction give strong chemiluminescence during their oxidation with N-bromosuccinimide (NBS) in alkaline solution. Humic acids from different sources produced similiar signal magnitude. This effect was employed to the flow-injection determination of glycine and arginine in pharmaceutical formulations with considerable selectivity against different amino compounds. The proposed method is fast and simple. Detection limit is 0.20 and 0.25 mg.l(-1) for glycine and arginine respectively, and 115 samples per h can be determined. (C) 2001 Elsevier Science B.V. All rights reserved.
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