Journal
BIOCHEMISTRY
Volume 53, Issue 10, Pages 1545-1547Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi401470u
Keywords
-
Categories
Funding
- Spanish MICINN [BFU2009-10185, BFU2012-32404, BFU2009-09738]
- ESFUNPROT (UCM)
Ask authors/readers for more resources
The ribosomal sarcin/ricin loop (SRL) is the target of ribosome-inactivating proteins like the N-glycosidase ricin and the fungal ribotoxin alpha-sarcin. The eukaryotic ribosomal stalk directly interacts with several members of the N-glycosidase family, favoring their disruption of the SRL. Here we tested this hypothesis for the ribotoxin alpha-sarcin. Experiments with isolated ribosomes, cell-free translation systems, and viability assays with Saccharomyces cerevisiae strains defective in acidic stalk proteins showed that the inactivation exerted by alpha-sarcin is independent of the composition of the ribosomal stalk. Therefore, alpha-sarcin, with the same ribosomal target as ricin, seems to access the SRL by a different pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available