Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 12, Issue 2, Pages 202-207Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00200-7
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Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. Improving renaturation yields by minimizing aggregation and reducing chemical costs are key to the industrial implementation of these processes. Recent developments include solubilization methods that do not rely on high denaturant concentrations and the use of high hydrostatic pressure for simultaneous solubilization and renaturation.
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