Journal
MOLECULAR CELL
Volume 7, Issue 4, Pages 741-751Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(01)00219-2
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Funding
- NCI NIH HHS [P30 CA08748] Funding Source: Medline
- NIGMS NIH HHS [T32-GM07464, GM60415] Funding Source: Medline
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Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30 Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable RscS/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.
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