Journal
BIOCHEMISTRY
Volume 52, Issue 31, Pages 5155-5157Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi4005655
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [22570120, 21113003]
- Grants-in-Aid for Scientific Research [22570120, 21113001, 21113003, 25116002] Funding Source: KAKEN
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It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.
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