Journal
BIOCHEMISTRY
Volume 52, Issue 17, Pages 2933-2948Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi400180d
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Funding
- National Institutes of Health (NIH) [R01EY05681]
- Research to Prevent Blindness
- National Center for Research Resources [5P41RR000954-35]
- National Institute of General Medical Sciences from the NIH [8 P41 GM103422-35]
- Washington University Institute of Clinical and Translational Sciences [UL1 TR000448]
- [EY02687]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR000448] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR000954] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [P30EY002687, R01EY005681] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103422] Funding Source: NIH RePORTER
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Mice with deletion of genes for small heat shock proteins alpha A- and alpha B-crystallin (alpha A/alpha B-/-) develop cataracts. We used proteomic analysis to identify lens proteins that change in abundance after deletion of these alpha-crystallin genes. Wild-type (WT) and alpha A/alpha B-1- knockout (DKO) mice were compared using two-dimensional difference gel electrophoresis and mass spectrometric analysis, and protein identifications were validated by Mascot proteomic software. The abundance of histones H2A, H4, and H2B fragment, and a low molecular weight beta 1-catenin increased 2-3-fold in postnatal day 2 lenses of DKO lenses compared with WT lenses. Additional major increases were observed in abundance of beta B2-crystallin and vimentin in 30-day-old lenses of DKO animals compared with WT animals. Lenses of DKO mice were comprised of nine protein spots containing beta B2-crystallin at 10-40-fold higher abundance and three protein spots containing vimentin at >= 2-fold higher abundance than in WT lenses. Gel permeation chromatography identified a unique 328 kDa protein in DKO lenses, containing beta-crystallin, demonstrating aggregation of beta-crystallin in the absence of alpha-crystallins. Together, these changes provide biochemical evidence for possible functions of specific cell adhesion proteins, cytoskeletal proteins, and crystallins in lens opacities caused by the absence of the. major chaperones, alpha A- and alpha B-crystallins.
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