Multidrug resistance is mediated by large plasmids carrying a class 1 integron in the emergent Salmonella enterica serotype [4,5,12:i:-]

A multidrug-resistant Salmonella enterica serotype [4,5,12:i:-] clone carried a class 1 integron harboring dfrA12. and aadA2 gene cassettes and bla(TEM-1), aac(3)-IV, cmlA1, and tetA genes located in large plasmids of about 140 kb (carrying spy) or 120 kb (lacking spy). Several segregants, lacking multidrug resistance, contained a plasmid smaller than the parental one and no longer hybridized with probes for the lost resistances. The genes mediating resistance to ampicillin, chloramphenicol, and tetracycline in the [4,5,12:i:-] clone are different from those found in the pentadrug-resistant serotype Typhimurium DT104 clone.