Development of an improved medium for germination of Cajanus cajan (L.) Millsp pollen in vitro

A simple, reliable medium for pollen germination of Cajanus cajan was developed by modifying Brewbaker and Kwack (BK) medium. Past attempts of C. cajan pollen germination in artificial media were not successful. A medium containing polyethylene glycol 4000 (PEG) showed more than 90% germination for C. cajan var. Pusa 33 only when the young buds (36 h before anthesis) were kept in pollen germination medium (PGM) for 36 h before pollen extraction. Supplementation of PGM with epsilon -amino caproic acid (EACA), an amino acid, showed improved pollen germination in Pusa 33 and also helped to avoid preconditioning of young buds before pollen extraction. It was also observed that there is a genotypic difference in the level of EACA required for in vitro pollen germination. Thus a complete medium for C. cajan genotypes consists of 37.5% sucrose + 15% PEG 4000 + 250 mg l(-1) boric acid + 300 mg l(-1) calcium nitrate + 100 mg l(-1) potassium nitrate + 200 mg l(-1) magnesium sulphate + 1% agar + EACA (0, 100, 250, 500, 750 or 1000 mg l(-1)).