Journal
BIOCHEMISTRY
Volume 51, Issue 49, Pages 9779-9781Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi301414q
Keywords
-
Categories
Funding
- Advanced Research Projects Agency-Energy (ARPA-E), U.S. Department of Energy [DE-AR0000091]
- National Science Foundation [EEC-0540879]
- Joint BioEnergy Institute
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
Ask authors/readers for more resources
Polyketides, an important class of natural products with, complex chemical structures, are widely used as antibiotics and other pharmaceutical agents. A clear barrier to heterologous polyketide biosynthesis in Escherichia coli is the lack of (2S)-methylmalonyl-CoA, a common substrate of multimodular polyketide synthases. Here we report a route for synthesizing (2S)-methylmalonyl-CoA from malonyl-CoA with a 3-hydroxypropionate cycle in thermoacidophilic crenarchaeon. The engineered E. coli strain produced both propionyl-CoA and methylmalonyl-CoA at intracellular levels similar to those of acetyl-CoA and succinyl-CoA, respectively. This approach may open a way to produce a variety of polyketide drugs in E. coli from renewable carbon sources.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available