Journal
BIOCHEMISTRY
Volume 51, Issue 13, Pages 2639-2641Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi300215k
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Funding
- NIH [GM55237]
- U.S. DOE, Office of Science, Office of Basic Energy Sciences [DE-AC02-06CH11357]
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Incorporation of a bicyclo[3.1.0]hexane scaffold into the nucleoside sugar was devised to lock the embedded cyclopentane ring in conformations that mimic the furanose North and South sugar puckers. To analyze the effects of North-methanocarba-2'-deoxythymidine (N-MCdT) on the B-form DNA, we crystallized d(CGCGAA[mcTmcT]CGCG) with two N-MCdTs. Instead of a duplex, the 12mer forms a tetraloop hairpin, whereby loop N-MCdTs adopt the C4'-exo pucker (NE; P = 50 degrees). Thus, the bicyclic framework does not limit the pucker to the anticipated C2'-exo range (NNW; P = 18 degrees).
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