Journal
JOURNAL OF NEUROSCIENCE
Volume 21, Issue 7, Pages 2288-2297Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.21-07-02288.2001
Keywords
axonal transport; glia; oligodendrocyte; myelin; shiverer; microtubule
Categories
Funding
- NIA NIH HHS [AG12646] Funding Source: Medline
- NINDS NIH HHS [R56 NS023868, NS23320, R01 NS023868, R01 NS023320, NS23868] Funding Source: Medline
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Altered axon-Schwann cell interactions in PNS myelin-deficient Trembler mice result in changed axonal transport rates, neurofilament and microtubule-associated protein phosphorylation, neurofilament density, and microtubule stability. To determine whether PNS and CNS myelination have equivalent effects on axons, neurofilaments, and microtubules in CNS, myelin-deficient shiverer axons were examined. The genetic defect in shiverer is a deletion in the myelin basic protein (MBP) gene, an essential component of CNS myelin. As a result, shiverer mice have little or no compact CNS myelin. Slow axonal transport rates in shiverer CNS axons were significantly increased, in contrast to the slowing in demyelinated PNS nerves. Even more striking were substantial changes in the composition and properties of microtubules in shiverer CNS axons. The density of axonal microtubules is increased, reflecting increased expression of tubulin in shiverer, and the stability of microtubules is drastically reduced in shiverer axons. Shiverer transgenic mice with two copies of a wild-type myelin basic protein transgene have an intermediate level of compact myelin, making it possible to determine whether the actual level of compact myelin is an important regulator of axonal microtubules. Both increased microtubule density and reduced microtubule stability were still observed in transgenic mouse nerves, indicating that signals beyond synaptogenesis and the mere presence of compact myelin are required for normal regulation of the axonal microtubule cytoskeleton.
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