Journal
BIOCHEMISTRY
Volume 50, Issue 33, Pages 7251-7258Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi200805j
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Funding
- BBSRC
- Cancer Research UK
- Trinity College
- BBSRC [BB/E019773/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E019773/1] Funding Source: researchfish
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G-Quadruplexes are noncanonical nucleic acid secondary structures based on guanine association that are readily adopted by G-rich RNA and DNA sequences. Naturally occurring genomic G-quadruplex-forming sequences have functional roles in biology that are mediated through structure. To appreciate how this is achieved, an understanding of the likelihood of G-quadruplex formation and the structural features of the folded species under a defined set of conditions of informative. We previously systematically investigted the thermodynamic stability and folding topology of DNA G-quadruplexes and found a strong dependence of these properties on loop length and loop arrangement [Bugaut, A., and Balasubramanian, S. (2008) Biochemistry 47, 689-697]. Here we report on a complementary analysis of RNA G-quadruplexes using UV melting and circular dichroism spectroscopy that also serves as a comparison to the equivalent DNA G-quadruplex-forming sequences. We found that the thermodynamic stability of G-quadruplex RNA can be modulated by loop length while the overall structure is largely unaffected. The systematic design of our study also revealed subtle loop length dependencies in RNA G-quadruplex structure.
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