Journal
JOURNAL OF INSECT PHYSIOLOGY
Volume 47, Issue 4-5, Pages 393-400Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0022-1910(00)00141-4
Keywords
pyrroline 5-carboxylate reductase; proline oxidase; cold tolerance; salt resistance; RNase protection assay
Categories
Ask authors/readers for more resources
Treatment of Drosophila melanogaster adults with an inhibitor of protein synthesis led to a decrease in intrinsic cold-shock tolerance, but no difference in the rapid cold hardening response, which is apparent only if a period at 4 degreesC precedes the cold stress. Increases in energy reserves, including proline, were found in lines of flies selected for resistance to chilling injury. Since an increase in proline levels has been associated with overwintering in insects, and for salt and cold tolerance in plants, an RNase protection assay was developed to assess changes in transcript abundance for two genes encoding enzymes important for proline metabolism, pyrroline 5-carboxylate reductase and proline oxidase. The mRNA levels did not change in response to low temperature, but the high level of pyrroline 5-carboxylate reductase transcript is consistent with the interpretation that a large proline pool is important for Drosophila metabolism and survival during cold stress. (C) 2001 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available